Posted in Resource, Yellow fever vaccine

The temporal transcriptomic profile of the YF17D vaccine

Windrose plots highlighting the blood transcriptomics module pathways that are increased (left) or decreased (right) that are modulated by YF17D vaccination. Source from Jue Hou et al., JI, 2017

The yellow fever live-attenuated vaccine is one of the most successful flaviviral vaccine developed. To better characterise the host responses involved, Jue Hou et al did a transcriptomic profiling of subjects vaccinated with YF17D across various time-intervals. The summary of the trial is as follows:

Study design:

  • 21 immunologically naive healthy subjects
  • Sampling at 4 hours, and days 1, 2, 3, 5, 7, 14, 28, 84 and 168 post-vaccination
  • Transcriptomics on PBMCs using Affymetrix arrays.

Main findings:

  • As early as 4 hours post-vaccination, 146 DEGs were detected. The number of DEGs peaked at day 2, and returned to baseline levels at day 28
  • B-cell population increased at day 14 vs day 0, and persisted. Mature B-cell population (IgD+ IgM+) increased after day 7 and remained at a high level. The IgM- only memory B cells (IgD-IgM+CD27+) showed a significant boost from day 28 to day 86
  • CD4+ and CD8+ T-cells expressing CD28 and Ki67 decreased at day 7, suggesting that T-cell proliferation probably occur at later time-points
  • Weighted correlation network analysis (WGCNA) highlights 4 main gene transcript modules most tightly associated with immunological signatures by YF17D
  • Raw file deposited at GSE82152

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