Have you ever wondered why some people respond better to vaccines than others? Using the live-attenuated YF17D vaccine, Querec et al investigated the molecular signatures induced by the vaccine, and the genes that are most associated with CD8 T-cells and antibody responses. The summary of the trial is as follows:
- 15 immunologically naive healthy subjects
- Sampling at days 0, 1, 3, 7 and 21 after vaccination
- Transcriptomics on PBMCs using Affymetrix Human Genome U133 Plus 2.0 Array
- Protein cytokine responses by 24-plex Luminex assay
- Only the chemokine IP-10 and the cytokine interleukin 1α (IL-1α) were significantly induced (P < 0.05), relative to day 0
- YF17D induces transcript expression of interferon, interferon-stimulated genes, complement and cell adhesion.
- Increased percentages of CD86+ myeloid DCs, plasmacytoid DCs, monocytes and CD14+CD16+ inflammatory monocytes at day 7 after vaccination
- Variations in CD8 T-cell and antibody responses between subjects differ by more than 10-fold, but differences not associated with IP10, IL1A and CD86 expression.
- At day 7, transcript levels of SLC2A6 (GLUT1), EIF2AK4 (GCN2), ITGAL, CTBP1 and FAM62B correlates with CD8 T-cell responses. At day 3, only YWHAE expression correlates with CD8 T-cell responses.
- At day 7, transcript levels of BEND4, PFKB3, TNFRSF7, TPD52, KBTBD7 and NAPIL2 correlates with neutralising antibody responses
- Raw data file deposited as GSE13486